Logo of University of Cologne

Qiagen Pyrosequencing

In brief the principle of pyrosequencing is a sequencing by synthesis. After incorporation of on ore more nucleotides of given type, pyrophosphate is generated whose degradation by a coupled enzyme assay produces a light flash, whose signal strength is proportional to the number of incorporated nucleotides.

What you can do with pyrosequencing is:

  • genotyping
  • methylation analysis
  • haplotype analysis

 

Pyrogram analysis software

 

The devices which operate in the CCG were previously marketed by the company Biotage. The pyrosequencing technology is used for the Roche 454 next generation sequencing system as well.

The CCG could evaluate pyrosequencing successfully for quantitative analysis and is therefore suitable for copy number detection.

PSQ 96MA

The system is now named PyroMark™Q96 ID.

 

PSQ96MA

Qiagen Pyrosequencer PSQ 96MA

 

PSQ HS96A

The system is now named PyroMark™Q96 MD.

 

PSQHS96A

Qiagen Pyrosequencer PSQ HS96A

 

 

Links